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Syntese av smittsomme Bacteriophages i en E. coli -basert Cell-free uttrykk System

Article DOI: 10.3791/56144-v • 11:33 min • August 17th, 2017
August 17th, 2017 •
1, 2, 1, 2, 1
1School of Physics and Astronomy, University of Minnesota, 2Department of Diagnostic and Biological Sciences and Institute for Molecular Virology, University of Minnesota

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En ny generasjon av celle-fri transkripsjon-oversettelse er konstruert for å konstruere biokjemiske systemer i vitro gjennom utførelsen av genet kretser. I denne artikkelen beskriver vi hvordan bacteriophages, som MS2, ΦΧ174 og T7, er synthesized fra sine genom bruker en alle E. coli cellen uten TXTL systemet.

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Bioteknologi problemet 126 Cell-free transkripsjon-oversettelse (TXTL) bacteriophage E. coli plakk analysen phage forsterkning phage genomet rensing syntetisk biologi
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