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JoVE Journal Developmental Biology
Integrationsfri afledning af humane inducerede pluripotente stamceller ved anvendelse af Laminin 521 Matrix
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Integrationsfri afledning af humane inducerede pluripotente stamceller ved anvendelse af Laminin 521 Matrix

Article DOI: 10.3791/56146-v • 10:48 min • July 7th, 2017
July 7th, 2017 •
1, 1,2, 1, 1, 1, 1
1Department of Neuroscience, Karolinska Institutet, 2Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet

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Robust afledning af humane inducerede pluripotente stamceller (hiPS) celler blev opnået ved anvendelse af ikke-integrerende Sendai virus (SeV) vektor-medieret omprogrammering af dermal fibroblaster. HiPS-cellevedligeholdelse og klonal ekspansion blev udført under anvendelse af xenofrie og kemisk definerede dyrkningsbetingelser med rekombinant human laminin 521 (LN-521) matrix og essentielt E8 (E8) medium.

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Udviklingsbiologi udgave 125 humane inducerede pluripotente stamceller omprogrammering Sendai virusvektorer fibroblaster Xeno-fri kemisk defineret rekombinant humant laminat 521
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