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Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains

DOI:
10.3791/56174-v

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10:13 min

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November 06, 2017

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, ,
1Program in Neuroscience,The College of Wooster, 2Department of Biology,The College of Wooster, 3Program in Biochemistry, Cellular, and Molecular Biology,The College of Wooster

Chapters

  • 00:05Title
  • 01:13Adult Brain Dissection
  • 03:39Brain Fixation and Immunofluorescent Staining
  • 06:09Mounting Adult D. melanogaster Brains onto Microscope Slides and Imaging
  • 07:47Results: Analyzing Axon Guidance Defects with the Mushroom Bodies
  • 09:04Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the dissection and immunostaining of adult Drosophila melanogaster brain tissues. Specifically, this protocol highlights the use of Drosophila mushroom body and photoreceptor neurons as example neuronal subsets that can be accurately used to uncover general principles underlying many aspects of neuronal development.

Tags

Keywords: Drosophila MelanogasterBrain DissectionImmunofluorescenceGFPDevelopmental BiologyNeuroscienceProtein LocalizationNeuronal ConnectivityBrain DevelopmentMorphological DefectsExoskeletonAnesthetized FliesPTNProboscisRetinaOptic LobeTrachea

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