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JoVE Journal Genetics
Probenvorbereitung und Analyse der RNASeq-basierte Genexpressionsdaten von Zebrafisch
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Probenvorbereitung und Analyse der RNASeq-basierte Genexpressionsdaten von Zebrafisch

Article DOI: 10.3791/56187-v • 11:42 min • October 27th, 2017
October 27th, 2017 •
*1, *1, 1
1Division of Endocrinology, Diabetes and Nutrition, Department of Medicine, University of Maryland School of Medicine
* These authors contributed equally

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Dieses Protokoll stellt einen Ansatz für die ganze Transkriptom-Analyse von Zebrafisch-Embryonen, Larven, oder Zellen sortiert. Wir gehören Isolierung von RNA, Pathway-Analyse von RNASeq Daten und qRT-PCR-basierte Validierung von Veränderungen der Genexpression.

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Ausgabe 128 Zebrafisch RNAseq Genetik Transkriptom RNA Danio Rerio Pfad-Analyse Gene Ontology Begriffe Strömung die Sortierung Fluoreszenz aktiviert Zellsortierung
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