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JoVE Journal Biochemistry
Enkel fjerning av bakgrunn fluorescens i Formalin-fast hjernevev for Immunofluorescence mikroskopi
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Enkel fjerning av bakgrunn fluorescens i Formalin-fast hjernevev for Immunofluorescence mikroskopi

Article DOI: 10.3791/56188-v • 16:31 min • September 3rd, 2017
September 3rd, 2017 •
1, 2, 1,2
1Department of Medical Biophysics, University of Toronto, 2Department of Biochemistry, University of Toronto

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Bakgrunn autofluorescence av biologiske prøver kompliserer ofte fluorescens-baserte Bildeteknikker, særlig i alderen menneskelig postmitotic vev. Denne protokollen beskriver hvordan autofluorescence fra disse prøvene effektivt kan fjernes ved hjelp av en kommersielt tilgjengelig lys emitting diode lys kilde photobleach prøven før immunostaining.

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Biokjemi problemet 127 Autofluorescence lipofuscin photobleaching lys emitting diode fluorescens mikroskopi immunofluorescence
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