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JoVE Journal
Genetics

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CRISPR/Cas9 和 SsODN 在人细胞中催化点突变修复功能的标准方法研究
 
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CRISPR/Cas9 和 SsODN 在人细胞中催化点突变修复功能的标准方法研究

Article DOI: 10.3791/56195
August 25th, 2017

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Summary August 25th, 2017

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该协议概述了 CRISPR/Cas9-based 基因编辑系统的工作流程, 用于修复哺乳动物细胞的点突变。在这里, 我们使用一个组合的方法来基因编辑和详细的后续实验策略, 测量 indel 形成在目标 site-in 本质, 分析现场诱变。

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