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Journal / Genetics / CRISPR/Cas9 和 SsODN…
JoVE Journal
Genetics

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JoVE Journal Genetics
CRISPR/Cas9 和 SsODN 在人细胞中催化点突变修复功能的标准方法研究
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CRISPR/Cas9 和 SsODN 在人细胞中催化点突变修复功能的标准方法研究

Article DOI: 10.3791/56195 • 10:08 min • August 25th, 2017
August 25th, 2017 •
1,2, 1,2
1Gene Editing Institute, Helen F. Graham Cancer Center and Research Institute, Christiana Care Health Services, 2Department of Medical Sciences, University of Delaware

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该协议概述了 CRISPR/Cas9-based 基因编辑系统的工作流程, 用于修复哺乳动物细胞的点突变。在这里, 我们使用一个组合的方法来基因编辑和详细的后续实验策略, 测量 indel 形成在目标 site-in 本质, 分析现场诱变。

Tags

分子生物学 问题 126 诱变 点突变修复 CRISPR/Cas9 单 DNA 寡核苷酸 基因编辑
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