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Journal / Biochemistry / Dissection de la rétine humaine et pre-choroïde…
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JoVE Journal Biochemistry
Dissection de la rétine humaine et pre-choroïde pour l’analyse protéomique
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Dissection de la rétine humaine et pre-choroïde pour l’analyse protéomique

Article DOI: 10.3791/56203-v • 06:55 min • November 12th, 2017
November 12th, 2017 •
*1,2,7,8, *3,4, 3,4, 1,2, 3, 3, 1,2, 5,6, 3,9
1Barbara & Donald Jonas Stem Cell Laboratory, and Bernard & Shirlee Brown Glaucoma Laboratory, Department of Pathology & Cell Biology, Institute of Human Nutrition, College of Physicians and Surgeons, Columbia University, 2Edward S. Harkness Eye Institute, New York-Presbyterian Hospital, 3Omics Laboratory, Byers Eye Institute, Department of Ophthalmology, Stanford University, 4Medical Scientist Training Program, University of Iowa, 5Department of Pediatrics, University of Iowa, 6Department of Neurology, University of Iowa, 7Department of Ophthalmology, Federal University of Sao Paulo (UNIFESP), 8Department of Ophthalmology, Federal University of EspÍrito Santo (UFES), 9Palo Alto Veterans Administration, Palo Alto, CA
* These authors contributed equally

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La rétine humaine est composée de régions fonctionnellement et moléculairement distinctes, y compris la fovéa, la macula et la rétine périphérique. Nous décrivons ici une méthode à l’aide de biopsies punch et élimination manuelle des couches de tissu d’un œil humain de disséquer et recueillir ces régions rétiniennes distinctes pour l’analyse protéomique en aval.

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Biochimie numéro 129 homme rétine dissection épithélium pigmentaire rétinien choroïde protéomique
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