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Journal / Biochemistry / Dissecação da Retina humana e RPE coroide para…
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Biochemistry
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JoVE Journal Biochemistry
Dissecação da Retina humana e RPE coroide para análise proteômica
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Dissecação da Retina humana e RPE coroide para análise proteômica

Article DOI: 10.3791/56203-v • 06:55 min • November 12th, 2017
November 12th, 2017 •
*1,2,7,8, *3,4, 3,4, 1,2, 3, 3, 1,2, 5,6, 3,9
1Barbara & Donald Jonas Stem Cell Laboratory, and Bernard & Shirlee Brown Glaucoma Laboratory, Department of Pathology & Cell Biology, Institute of Human Nutrition, College of Physicians and Surgeons, Columbia University, 2Edward S. Harkness Eye Institute, New York-Presbyterian Hospital, 3Omics Laboratory, Byers Eye Institute, Department of Ophthalmology, Stanford University, 4Medical Scientist Training Program, University of Iowa, 5Department of Pediatrics, University of Iowa, 6Department of Neurology, University of Iowa, 7Department of Ophthalmology, Federal University of Sao Paulo (UNIFESP), 8Department of Ophthalmology, Federal University of EspÍrito Santo (UFES), 9Palo Alto Veterans Administration, Palo Alto, CA
* These authors contributed equally

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A retina humana é composta de regiões funcionalmente e molecularmente distintas, incluindo a fóvea, mácula e retina periférica. Aqui, descrevemos um método usando o soco de biópsias e remoção manual das camadas de tecido do olho humano para dissecar e coletar estas regiões distintas da retina para análise proteômica a jusante.

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Bioquímica edição 129 humanos retina dissecação Epitélio pigmentado da retina coroide proteomics
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