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AFM e Microrheology na célula de embrião de Zebrafish gema
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AFM e Microrheology na célula de embrião de Zebrafish gema

Article DOI: 10.3791/56224-v • 09:48 min • November 29th, 2017
November 29th, 2017 •
1, 2, 1, 2, 2, 1
1Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, 2Institute for Bioengineering of Catalonia, Universitat de Barcelona and CIBER Enfermedades Respiratorias

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A falta de ferramentas para medir propriedades materiais e parâmetros tensional na vivo impede Validando seus papéis durante o desenvolvimento. Utilizamos microscopia de força atômica (AFM) e acompanhamento de nanopartículas para quantificar as características mecânicas da célula de gema intacta zebrafish embrião durante epiboly. Esses métodos são confiáveis e amplamente aplicável, evitando intervenções intrusivas.

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Biologia do desenvolvimento edição 129 Zebrafish gema microscopia de força atômica tensão Cortical Microrheology nanopartículas de rastreamento
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