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Biochemistry

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JoVE Journal Biochemistry
DNA Polymerase aktivitet Assay ved hjælp af nær-infrarødt fluorescerende mærket DNA visualiseret af acrylamid Gel elektroforese
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DNA Polymerase aktivitet Assay ved hjælp af nær-infrarødt fluorescerende mærket DNA visualiseret af acrylamid Gel elektroforese

Article DOI: 10.3791/56228-v • 07:38 min • October 6th, 2017
October 6th, 2017 •
1, 1
1Department of Chemistry, W.M. Keck Science Department of Claremont Mckenna, Pitzer, and Scripps College

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Denne protokol beskriver karakterisering af DNA polymerase syntese af modificeret DNA gennem observation af ændringer til nær-infrarødt fluorescently mærket DNA ved hjælp af gelelektroforese og gel billeddannelse. Acrylamid geler bruges til høj opløsning billeddannelse af adskillelse af korte nukleinsyrer, der overflyttes til forskellige priser afhængig af størrelse.

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Biokemi spørgsmålet 128 DNA polymerase engineering DNA polymerase assay gelelektroforese modificeret DNA protein engineering Michaelis-Menten kinetik af DNA polymeraser DNA polymeraser
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