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Journal / Neuroscience / Visualisering og Live Imaging af Oligodendrocyte…
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Neuroscience

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JoVE Journal Neuroscience
Visualisering og Live Imaging af Oligodendrocyte organeller i Organotypic hjerne skiver ved hjælp af Adeno-associeret Virus og konfokal mikroskopi
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Visualisering og Live Imaging af Oligodendrocyte organeller i Organotypic hjerne skiver ved hjælp af Adeno-associeret Virus og konfokal mikroskopi

Article DOI: 10.3791/56237-v • 08:38 min • October 23rd, 2017
October 23rd, 2017 •
1, 1,2
1Division of Anatomy, Institute of Basic Medical Sciences, University of Oslo, 2Department of Microbiology, Oslo University Hospital

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Myelinating oligodendrocytes fremme hurtig aktionspotentialet formering og neuronal overlevelse. Beskrevet her er en protokol til oligodendrocyte-specifikke udtryk af fluorescerende proteiner i organotypic hjerne skiver med efterfølgende time-lapse billeddannelse. Derudover præsenteres en enkel procedure til at visualisere unstained myelin.

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Neurovidenskab spørgsmålet 128 Time-lapse oligodendrocytes organotypic live imaging mitokondrier transduktion adeno-associeret virus myelin Konfokal Reflektionsgraden mikroskopi
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