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Bakken staten uttømming super-oppløsning Imaging i pattedyrceller
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Bakken staten uttømming super-oppløsning Imaging i pattedyrceller

Article DOI: 10.3791/56239-v • 07:55 min • November 5th, 2017
November 5th, 2017 •
1, 1, 1, 1
1Department of Physiology and Membrane Biology, University of California School of Medicine, Davis

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Denne artikkelen beskriver en protokoll for påvisning av én eller flere plasma og/eller intracellulær membran proteiner med bakken staten nedbryting (GSD) Super-oppløsning mikroskopi i pattedyrceller. Her diskuterer vi fordeler og betraktninger ved bruk av slike metoder for visualisering og kvantifisering av mobilnettet proteiner.

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Strukturell biologi problemet 129 CaV1.2 AC confocal mikroskopi endoplasmatiske retikulum (ER) super-oppløsning totalt interne refleksjon fluorescens (TIRF) mikroskopi spenning gated kalsium kanaler bakken staten uttømming mikroskopi etterfulgt av individuelle molekyl avkastning (GSDIM)
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