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Journal / Biochemistry / Regeneratie van gekleed gouden Microelectrodes…
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Biochemistry

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JoVE Journal Biochemistry
Regeneratie van gekleed gouden Microelectrodes uitgerust voor een Real-Time cel Analyzer
Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
Journal (Biochemistry)
Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
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Journal (Biochemistry)
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Regeneratie van gekleed gouden Microelectrodes uitgerust voor een Real-Time cel Analyzer

Article DOI: 10.3791/56250-v • 12:47 min • March 12th, 2018
March 12th, 2018 •
*1, *1, 2, 3, 3, 1, 4
1School of Public Health, Nanjing Medical University, 2School of Pharmacy, Nanjing Medical University, 3State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry and Chemical Engineering, Nanjing Tech University, 4Department of Medical Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University
* These authors contributed equally

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Dit protocol beschrijft een algemene strategie om te regenereren commerciële gekleed gouden microelectrodes uitgerust voor een label-vrije cel analyzer besparing op de hoge lopende kosten ofmicrochip gebaseerde tests gericht. Het regeneratieproces omvat de spijsvertering van de trypsine, spoelen met ethanol en water, en een stap van spinnen, waardoor herhaalde gebruik van microchips.

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Biochemie kwestie 133 Label-vrije cel-gebaseerde assay regeneratie gekleed gouden microelectrodes real-time cel analyzer trypsine
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