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Biochemistry

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JoVE Journal Biochemistry
Regeneración de microelectrodos de oro vestidas para un analizador en tiempo real de la célula
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Regeneración de microelectrodos de oro vestidas para un analizador en tiempo real de la célula

Article DOI: 10.3791/56250-v • 12:47 min • March 12th, 2018
March 12th, 2018 •
*1, *1, 2, 3, 3, 1, 4
1School of Public Health, Nanjing Medical University, 2School of Pharmacy, Nanjing Medical University, 3State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry and Chemical Engineering, Nanjing Tech University, 4Department of Medical Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University
* These authors contributed equally

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Este protocolo describe una estrategia general para regenerar comerciales microelectrodos de oro vestidas para un analizador de célula libre de etiqueta para ahorro en los alta ejecución costos ofmicrochip-ensayos. El proceso de regeneración incluye la digestión de la tripsina, enjuague con agua y etanol y un paso de giro, que permite el uso repetido de los microchips.

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Ensayo de etiqueta-libre celular de bioquímica número 133 regeneración v. microelectrodos oro analizador del celular en tiempo real tripsina
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