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Biochemistry

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JoVE Journal Biochemistry
Regenerering af klædt guld Microelectrodes udstyret til en Real-Time celle Analyzer
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Regenerering af klædt guld Microelectrodes udstyret til en Real-Time celle Analyzer

Article DOI: 10.3791/56250-v • 12:47 min • March 12th, 2018
March 12th, 2018 •
*1, *1, 2, 3, 3, 1, 4
1School of Public Health, Nanjing Medical University, 2School of Pharmacy, Nanjing Medical University, 3State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry and Chemical Engineering, Nanjing Tech University, 4Department of Medical Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University
* These authors contributed equally

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Denne protokol beskriver en generel strategi for at regenerere kommercielle klædt guld microelectrodes udstyret til en etiket-gratis celle analyzer sigter besparelse på de høje løbende omkostninger ofmicrochip-baserede assays. Regenereringsprocessen omfatter trypsin fordøjelse, skylning med ethanol og vand og en spinning skridt, som giver mulighed for gentagen brug af mikrochips.

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Biokemi sag 133 etiket-fri celle-baserede assay regenerering iført guld microelectrodes real-time celle analyzer trypsin
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