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Journal / Bioengineering / Efficient Generation and Editing of Feeder-free…
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Bioengineering

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JoVE Journal Bioengineering
Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System
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Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System

Article DOI: 10.3791/56260-v • 09:16 min • November 8th, 2017
November 8th, 2017 •
1,2, 3, 1,2,4
1Department of Animal and Avian Sciences, University of Maryland, 2Animal Bioscience and Biotechnology Laboratory, ARS, USDA, 3NIH Stem Cell Unit, Bethesda, National Institutes of Health, 4RenOVAte Biosciences Inc

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Summary
Automatic Translation

This protocol describes in detail the generation of footprint-free induced pluripotent stem cells (iPSCs) from human pancreatic cells in feeder-free conditions, followed by editing using CRISPR/Cas9 ribonucleoproteins and characterization of the modified single-cell clones.

Tags

Feeder-free IPSCs Human Pancreatic Cells CRISPR-Cas9 System Footprint-free IPSCs Genome Editing CRISPR-Cas9 Ribonucleoproteins Single Cell Clones Clonal Lines Mosaicism Collagen Coating Prigrow III Medium Transduction Reprogramming Virus Mixture
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