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Bioengineering

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JoVE Journal Bioengineering
Eficiente generación y edición de IPSCs alimentador-libre de las células pancreáticas humanas utilizando el sistema CRISPR Cas9
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Eficiente generación y edición de IPSCs alimentador-libre de las células pancreáticas humanas utilizando el sistema CRISPR Cas9

Article DOI: 10.3791/56260-v • 09:16 min • November 8th, 2017
November 8th, 2017 •
1,2, 3, 1,2,4
1Department of Animal and Avian Sciences, University of Maryland, 2Animal Bioscience and Biotechnology Laboratory, ARS, USDA, 3NIH Stem Cell Unit, Bethesda, National Institutes of Health, 4RenOVAte Biosciences Inc

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Este protocolo se describe en detalle la generación de células libres de huella pluripotentes inducidas (iPSCs) de las células pancreáticas humanas en condiciones libres de alimentador, seguido de edición utilizando ribonucleoproteínas CRISPR/Cas9 y caracterización de la modificación sola célula clones.

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Bioingeniería número 129 inducida de pluripotent células (iPSCs) reprogramación virus sendai iPSCs CRISPR/Cas9 libres de huella nucleofection
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