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JoVE Journal Bioengineering
Produktion af gensplejsede gyldne syriske hamstere af Pronuclear injektion af CRISPR/Cas9-komplekset
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Produktion af gensplejsede gyldne syriske hamstere af Pronuclear injektion af CRISPR/Cas9-komplekset

Article DOI: 10.3791/56263-v • 06:12 min • January 9th, 2018
January 9th, 2018 •
*1, *1,2, 1, 3, 3,4, 2,5, 1
1Department of Animal, Dairy, and Veterinary Sciences, Utah State University, 2National Centre for International Research in Cell and Gene Therapy, Sino-British Research Centre, School of Basic Medical Sciences, Zhengzhou University, 3Department of Animal Science Division of Applied Life Science (BK21 Plus), Gyeongsang National University, 4Institute of Agriculture and Life Science, Gyeongsang National University, 5Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London
* These authors contributed equally

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Pronuclear (PN) injektion af den grupperede regelmæssigt interspaced korte palindromiske gentagelser (CRISPR) og CRISPR-forbundet protein-9 nukleasen (CRISPR/Cas9) system er en yderst effektiv metode til fremstilling af gensplejsede gyldne syriske hamstere. Heri, beskriver vi detaljeret PN injektion protokollen til produktion af genet knockout hamstere med CRISPR/Cas9-systemet.

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Bioteknologi sag 131 Pronuclear injektion CRISPR/Cas9 gen redigering knockout zygote ægoplægningen dyremodel gyldne syriske hamster
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