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Journal / Bioengineering / Production de génétiquement Hamsters syriens…
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Bioengineering

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JoVE Journal Bioengineering
Production de génétiquement Hamsters syriens dorés par Injection pronucléaire du complexe CRISPR/Cas9
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Production de génétiquement Hamsters syriens dorés par Injection pronucléaire du complexe CRISPR/Cas9

Article DOI: 10.3791/56263-v • 06:12 min • January 9th, 2018
January 9th, 2018 •
*1, *1,2, 1, 3, 3,4, 2,5, 1
1Department of Animal, Dairy, and Veterinary Sciences, Utah State University, 2National Centre for International Research in Cell and Gene Therapy, Sino-British Research Centre, School of Basic Medical Sciences, Zhengzhou University, 3Department of Animal Science Division of Applied Life Science (BK21 Plus), Gyeongsang National University, 4Institute of Agriculture and Life Science, Gyeongsang National University, 5Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London
* These authors contributed equally

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Pronucléaire injection (PN) du cluster régulièrement entrecoupées courtes répétitions palindromiques (CRISPR) et le système CRISPR-associated protein-9 nucléase (CRISPR/Cas9) est une méthode très efficace pour produire génétiquement hamsters syriens dorés. Ici, les auteurs décrivent le protocole détaillé d’injection de PN pour la production des hamsters de knock-out du gène avec le système CRISPR/Cas9.

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Bio-ingénierie numéro 131 injection pronucléaire CRISPR/Cas9 zygote de montage le knockout gène transfert d’embryons modèle animal hamster doré de Syrie
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