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Journal / Bioengineering / Üretim CRISPR/Cas9 kompleks Pronuclear…
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JoVE Journal Bioengineering
Üretim CRISPR/Cas9 kompleks Pronuclear enjeksiyonla genetiği altın Suriye hamster
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Üretim CRISPR/Cas9 kompleks Pronuclear enjeksiyonla genetiği altın Suriye hamster

Article DOI: 10.3791/56263-v • 06:12 min • January 9th, 2018
January 9th, 2018 •
*1, *1,2, 1, 3, 3,4, 2,5, 1
1Department of Animal, Dairy, and Veterinary Sciences, Utah State University, 2National Centre for International Research in Cell and Gene Therapy, Sino-British Research Centre, School of Basic Medical Sciences, Zhengzhou University, 3Department of Animal Science Division of Applied Life Science (BK21 Plus), Gyeongsang National University, 4Institute of Agriculture and Life Science, Gyeongsang National University, 5Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London
* These authors contributed equally

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Pronuclear (PN) iğne kümelenmiş düzenli olarak kısa palindromik yineler (CRISPR) interspaced ve CRISPR ilişkili protein-9 nükleaz (CRISPR/Cas9) sistemi genetiği altın Suriye hamster üretmek için son derece verimli bir yöntemdir. Burada, biz gene nakavt hamster CRISPR/Cas9 sistemi ile üretim için detaylı PN enjeksiyon Protokolü tanımlamak.

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Biyomühendislik sayı: 131 Pronuclear enjeksiyon CRISPR/Cas9 gen düzenleme nakavt zigot embriyo transferi hayvan modeli altın Suriye hamster
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