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Journal / Bioengineering / Produção de geneticamente modificados Hamsters…
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Bioengineering

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JoVE Journal Bioengineering
Produção de geneticamente modificados Hamsters sírios dourados por injeção pró-nuclear do complexo CRISPR/Cas9
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A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
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Produção de geneticamente modificados Hamsters sírios dourados por injeção pró-nuclear do complexo CRISPR/Cas9

Article DOI: 10.3791/56263-v • 06:12 min • January 9th, 2018
January 9th, 2018 •
*1, *1,2, 1, 3, 3,4, 2,5, 1
1Department of Animal, Dairy, and Veterinary Sciences, Utah State University, 2National Centre for International Research in Cell and Gene Therapy, Sino-British Research Centre, School of Basic Medical Sciences, Zhengzhou University, 3Department of Animal Science Division of Applied Life Science (BK21 Plus), Gyeongsang National University, 4Institute of Agriculture and Life Science, Gyeongsang National University, 5Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London
* These authors contributed equally

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Pró-nuclear injeção (PN) do cluster regularmente intercaladas curtas palíndromos repetições (CRISPR) e sistema CRISPR-associada da proteína-9 nuclease (CRISPR/Cas9) é um método altamente eficiente para produzir hamster sírio dourado geneticamente modificado. Aqui, descrevemos o protocolo de injeção PN detalhado para a produção de hamsters de gene nocaute com o sistema CRISPR/Cas9.

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Bioengenharia questão 131 injeção pró-nuclear CRISPR/Cas9 zigoto de edição nocaute gene transferência de embriões modelo animal hamster sírio dourado
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