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Bioengineering

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JoVE Journal Bioengineering
Produktion av genmanipulerade gyllene syriska hamstrar genom pronukleär injektion av CRISPR/Cas9 komplexet
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Produktion av genmanipulerade gyllene syriska hamstrar genom pronukleär injektion av CRISPR/Cas9 komplexet

Article DOI: 10.3791/56263-v • 06:12 min • January 9th, 2018
January 9th, 2018 •
*1, *1,2, 1, 3, 3,4, 2,5, 1
1Department of Animal, Dairy, and Veterinary Sciences, Utah State University, 2National Centre for International Research in Cell and Gene Therapy, Sino-British Research Centre, School of Basic Medical Sciences, Zhengzhou University, 3Department of Animal Science Division of Applied Life Science (BK21 Plus), Gyeongsang National University, 4Institute of Agriculture and Life Science, Gyeongsang National University, 5Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London
* These authors contributed equally

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Pronukleär (PN) injektion av den klustrade regelbundet mellanliggande kort palindromic repetitioner (CRISPR) och CRISPR-associerade protein-9 nuclease (CRISPR/Cas9) system är en mycket effektiv metod för att producera genetiskt modifierade gyllene syriska hamstrar. Häri, beskriver vi detaljerad PN injektion protokollet för produktion av genen knockout hamstrar med CRISPR/Cas9-systemet.

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Bioteknik fråga 131 pronukleär injektion gen fotoredigering knockout zygot djurmodell embryotransfer CRISPR/Cas9 gyllene syriska hamster
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