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Cancer Research

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JoVE Journal Cancer Research
Anvendelsen af Laser mikro-bestråling for undersøgelse af enkelt og dobbelt Strand bryde reparation i pattedyrceller
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Anvendelsen af Laser mikro-bestråling for undersøgelse af enkelt og dobbelt Strand bryde reparation i pattedyrceller

Article DOI: 10.3791/56265-v • 08:18 min • September 5th, 2017
September 5th, 2017 •
1, 1, 1
1Department of Oncologic Sciences, University of South Alabama Mitchell Cancer Institute

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Konfokal Fluorescens mikroskopi og laser mikro-bestråling tilbyder værktøjer til inducerende DNA-skader og overvåge svaret fra DNA reparation proteiner i udvalgte sub nukleare områder. Denne teknik har betydeligt avancerede vores kendskab til skader påvisning, signalering og rekruttering. Dette manuskript viser disse teknologier for at undersøge enkelt og dobbelt strand bryde reparation.

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Kræftforskning sag 127 DNA reparation laser mikro-bestråling DNA-skader strand pause XRCC1 gamma-H2AX strand bryde signalering
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