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Journal / Genetics / Microinjeção de proteína CRISPR/Cas9 em bagre do…
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Genetics
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Microinjeção de proteína CRISPR/Cas9 em bagre do canal, Ictalurus punctatus, embriões para Gene Editing
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Microinjeção de proteína CRISPR/Cas9 em bagre do canal, Ictalurus punctatus, embriões para Gene Editing

Article DOI: 10.3791/56275-v • 16:08 min • January 20th, 2018
January 20th, 2018 •
*1,2, *1,3, 1, 4, 4, 5, 5, 1
1School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, 2Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, 3Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, 4Department of Molecular Physiology and Biophysics, Vanderbilt University, 5Life Science Institute, University of Michigan
* These authors contributed equally

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Um protocolo simples e eficiente de microinjeção para edição de genes em embriões de bagre de canal usando o sistema CRISPR/Cas9 é apresentado. Neste protocolo, guia de RNAs e proteínas Cas9 foram injetadas na gema de uma célula de embriões. Este protocolo foi validado por nocauteando dois genes imune-relacionados de bagre do canal.

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Genética questão 131 CRISPR/Cas9 gene edição nocaute do gene microinjeção embriões de bagre do canal mutação truncado proteína puntuais
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