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Journal / Genetics / Microinyección de la proteína CRISPR/Cas9 en…
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Genetics
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JoVE Journal Genetics
Microinyección de la proteína CRISPR/Cas9 en bagre de canal, Ictalurus punctatus, embriones de Gene Editing
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Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation
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Journal (Genetics)
A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
 
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Microinyección de la proteína CRISPR/Cas9 en bagre de canal, Ictalurus punctatus, embriones de Gene Editing

Article DOI: 10.3791/56275-v • 16:08 min • January 20th, 2018
January 20th, 2018 •
*1,2, *1,3, 1, 4, 4, 5, 5, 1
1School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, 2Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, 3Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, 4Department of Molecular Physiology and Biophysics, Vanderbilt University, 5Life Science Institute, University of Michigan
* These authors contributed equally

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Se presenta un protocolo de microinyección simple y eficiente para la edición de genes en embriones de bagre de canal usando el sistema CRISPR/Cas9. En este protocolo, guía RNAs y proteínas Cas9 fueron microinyectados en la yema de una célula de embriones. Este protocolo ha sido validada por la anulación de dos genes relacionados con la inmune de bagre de canal.

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Genética número 131 CRISPR/Cas9 edición microinyección embriones de bagre de canal nocaut de gen mutación gen proteína indels truncada
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