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JoVE Journal
Genetics

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JoVE Journal Genetics
Hybridation fluorescente In Situ haute résolution dans des embryons de drosophile et tissus à l’aide d’Amplification de Signal Tyramide
High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology
Journal (Genetics)
High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology
Differentiation, Maintenance, and Analysis of Human Retinal Pigment Epithelium Cells: A Disease-in-a-dish Model for BEST1 Mutations
Journal (Genetics)
Differentiation, Maintenance, and Analysis of Human Retinal Pigment Epithelium Cells: A Disease-in-a-dish Model for BEST1 Mutations
 
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Hybridation fluorescente In Situ haute résolution dans des embryons de drosophile et tissus à l’aide d’Amplification de Signal Tyramide

Article DOI: 10.3791/56281
October 19th, 2017 •
1,2, 1, 1,2, 1,2
1The Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 2Department of Molecular Genetics, University of Toronto

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October 19th, 2017

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Le protocole d’ARN décrit in situ hybridation permet la détection de l’ARN dans des embryons de drosophile entiers ou tissus disséqués. À l’aide de microplaques 96 puits et amplification de signal tyramide, transcriptions peuvent être détectées à un débit, sensibilité et haute résolution et à un coût relativement faible.

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