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Journal / Genetics / Høy oppløsning fluorescerende In Situ…
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Genetics

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JoVE Journal Genetics
Høy oppløsning fluorescerende In Situ hybridisering i Drosophila embryoer og vev med Tyramide Signal forsterkning
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Høy oppløsning fluorescerende In Situ hybridisering i Drosophila embryoer og vev med Tyramide Signal forsterkning

Article DOI: 10.3791/56281-v • 14:21 min • October 19th, 2017
October 19th, 2017 •
1,2, 1, 1,2, 1,2
1The Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 2Department of Molecular Genetics, University of Toronto

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Beskrevet RNA i situ hybridisering protokollen tillater påvisning av RNA i hele Drosophila embryo eller dissekert vev. Bruke 96-brønnen microtiter plater og tyramide signalforsterkning, kan utskrifter oppdages ved høy oppløsning og følsomhet gjennomstrømming og en relativt lav kostnad.

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Genetikk problemet 128 RNA subcellular lokalisering lysrør i situ hybridisering fisk Drosophila høy gjennomstrømming tyramide signalforsterkning fosteret vev.
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