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Journal / Genetics / 高分辨率荧光原位杂交在果蝇胚胎和组织中使用 Tyramide…
JoVE Journal
Genetics

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JoVE Journal Genetics
高分辨率荧光原位杂交在果蝇胚胎和组织中使用 Tyramide 信号放大
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高分辨率荧光原位杂交在果蝇胚胎和组织中使用 Tyramide 信号放大

Article DOI: 10.3791/56281-v • 14:21 min • October 19th, 2017
October 19th, 2017 •
1,2, 1, 1,2, 1,2
1The Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 2Department of Molecular Genetics, University of Toronto

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所描述的 rna原位杂交协议允许检测整个果蝇胚胎或解剖组织中的 rna。使用96井孔板和 tyramide 信号放大, 记录可以检测高分辨率, 灵敏度和吞吐量, 并以较低的成本。

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遗传学 问题 128 RNA 亚细胞定位 荧光原位杂交 鱼 果蝇 高通量 tyramide 信号放大 胚胎 组织。
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