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Immunology and Infection

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JoVE Journal Immunology and Infection
Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy
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Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy

Article DOI: 10.3791/56284-v • 09:24 min • November 29th, 2017
November 29th, 2017 •
1, 1, 1, 2, 1
1EPSRC Proteus Hub, MRC Centre of Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, 2School of Chemistry, EaStChem, University of Edinburgh

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Summary
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This technique describes an efficient screening process for evaluating bacteria-specific optical imaging agents within ex vivo human lung tissue, by fibered confocal fluorescence microscopy for the rapid identification of small molecule chemical probe-candidates with translatable potential.

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Optical Screening Novel Bacteria-specific Probes Ex Vivo Human Lung Tissue Confocal Laser Endomicroscopy Molecular Imaging Translatable Technique Imaging Probes Clinic Equipment Human Lung Tissue Sample Thawing Tissue Slicing Tissue Tissue Culture Plate Washing Sample Calcein A Staining
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