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Journal / Biochemistry / Biochemische und strukturelle Charakterisierung…
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Biochemistry

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JoVE Journal Biochemistry
Biochemische und strukturelle Charakterisierung von Kohlenhydrat Transport Substrat-Bindeprotein SP0092
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Biochemische und strukturelle Charakterisierung von Kohlenhydrat Transport Substrat-Bindeprotein SP0092

Article DOI: 10.3791/56294-v • 08:53 min • October 2nd, 2017
October 2nd, 2017 •
1,2, 1,2, 1, 1,2
1Diamond Light Source, Harwell Science & Innovation Campus, 2Research Complex at Harwell, Harwell Science & Innovation Campus

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Eine optimierte Protokoll für die Durchführung einer umfangreichen biochemischen und strukturelle Charakterisierung von ein Kohlenhydrat-Substrat-Bindeprotein von Streptococcus Pneumoniae wird vorgestellt.

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Biochemie Ausgabe 128 ABC-Transporter Streptococcus Pneumoniae Kohlenhydrat-Aufnahme Substrat-Bindeprotein thermische Verschiebung Assay Multi Winkel Lichtstreuung (MALS) Größe Ausgrenzung Chromatography (SEC) Kristallographie x-ray Beugung Datenerfassung Proteinlösung Struktur
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