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Immunology and Infection

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JoVE Journal Immunology and Infection
Målretning cystein dithioler for in Vitro lokationsspecifikke glykosylering af rekombinante proteiner
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Målretning cystein dithioler for in Vitro lokationsspecifikke glykosylering af rekombinante proteiner

Article DOI: 10.3791/56302-v • 11:25 min • October 4th, 2017
October 4th, 2017 •
*1, *1, *1, *1, 1, 1
1Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario
* These authors contributed equally

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Biokemiske og strukturelle analyser af glykosyleret proteiner kræver relativt store mængder af homogene prøver. Her præsenterer vi en effektiv kemisk metode for lokationsspecifikke glykosylering af rekombinante proteiner renset fra bakterier ved at målrette reaktive Cys dithioler.

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Immunologi spørgsmålet 128 glykosylering methanethiosulfonate cystein thiol modifikation stromale interaktion molekyle-1 EFSAM løsning Kernemagnetisk resonans-spektroskopi massespektrometri
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