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Journal / Immunology and Infection / Cysteïne thiolen richtend voor in Vitro…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Cysteïne thiolen richtend voor in Vitro site-specifieke glycosylatie van recombinante eiwitten
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Cysteïne thiolen richtend voor in Vitro site-specifieke glycosylatie van recombinante eiwitten

Article DOI: 10.3791/56302-v • 11:25 min • October 4th, 2017
October 4th, 2017 •
*1, *1, *1, *1, 1, 1
1Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario
* These authors contributed equally

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Biochemische en structurele analyses van geglycosyleerde eiwitten vereist relatief grote hoeveelheden van homogene monsters. Hier presenteren we een efficiënte chemische methode voor site-specifieke glycosylatie van recombinante eiwitten gezuiverd van bacteriën door zich te richten van reactieve Cys thiolen.

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Immunologie kwestie 128 glycosylation methanethiosulfonate cysteïne thiol wijziging stromale interactie molecuul-1 EFSAM oplossing nucleaire magnetische resonantie spectroscopie massaspectrometrie
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