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Journal / Immunology and Infection / Targeting Cystein Thiole für in-vitro-…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Targeting Cystein Thiole für in-vitro- standortspezifische Glykosylierung von rekombinanten Proteinen
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Targeting Cystein Thiole für in-vitro- standortspezifische Glykosylierung von rekombinanten Proteinen

Article DOI: 10.3791/56302-v • 11:25 min • October 4th, 2017
October 4th, 2017 •
*1, *1, *1, *1, 1, 1
1Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario
* These authors contributed equally

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Biochemische und strukturelle Analysen des glykosylierten Proteinen erfordern relativ große Mengen an homogenen Proben. Hier präsentieren wir Ihnen eine effiziente chemische Methode für standortspezifische Glykosylierung von rekombinanten Proteinen durch gezielte reaktive Cys Thiole von Bakterien gereinigt.

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Ausgabe 128 Glykosylierung Methanethiosulfonate Cystein Thiol-Modifikation Immunologie stromale Interaktion Molekül-1 EFSAM Lösung magnetischen Kernresonanz-Spektroskopie Massenspektrometrie
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