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JoVE Journal Genetics
Kortlægning Genome-wide tilgængelige kromatin i primære humane T lymfocytter af ATAC-Seq
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Kortlægning Genome-wide tilgængelige kromatin i primære humane T lymfocytter af ATAC-Seq

Article DOI: 10.3791/56313-v • 09:08 min • November 13th, 2017
November 13th, 2017 •
1, 1, 1, 1, 1
1The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University

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Assay for Transposase-tilgængelige kromatin kombineret med høj overførselshastighed sekventering (ATAC-FF.) er en genome-wide metode til at afdække tilgængelige kromatin. Dette er en trinvis ATAC-seq-protokollen fra molekylære til den endelige beregningsmæssige analyse, optimeret til humane lymfocytter (Th1/Th2). Denne protokol kan vedtages af forskere uden forudgående erfaring i næste generations sekventering metoder.

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Genetik sag 129 kromatin tilgængelighed ATAC-FF. menneskelige CD4 + lymfocytter næste generation sequencing regulerende elementer enhancer initiativtageren Th1 Th2
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