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Journal / Developmental Biology / Metoder for Imaging intracellulær pH i hårsekken…
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Developmental Biology

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JoVE Journal Developmental Biology
Metoder for Imaging intracellulær pH i hårsekken stamcelleforskningen slektslinje i Live Drosophila Ovarian vev
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Metoder for Imaging intracellulær pH i hårsekken stamcelleforskningen slektslinje i Live Drosophila Ovarian vev

Article DOI: 10.3791/56316-v • 08:05 min • September 26th, 2017
September 26th, 2017 •
1, 1, 1
1Departments of Anatomy and OB/GYN-RS, University of California, San Francisco

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Vi tilbyr en protokoll for imaging intracellulær pH i en epithelial stamcelleforskningen slektslinje i levende Drosophila ovarian vev. Vi beskriver metoder for å generere transgene fluer uttrykke en pH biosensor, mCherry::pHluorin, bilde biosensor med kvantitative fluorescens imaging, standard kurver genererer og konvertere fluorescens intensitetsverdiene til pH-verdier.

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Utviklingsbiologi problemet 127 intracellulære pH hårsekken stilk cellen tarmepitelet celledifferensiering pHluorin kvantitativ fluorescens bildebehandling
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