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Journal / Developmental Biology / Metoder til billeddannelse intracellulære pH af…
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Developmental Biology

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JoVE Journal Developmental Biology
Metoder til billeddannelse intracellulære pH af follikel stamcelle afstamning i Live Drosophila æggestokkene væv
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Metoder til billeddannelse intracellulære pH af follikel stamcelle afstamning i Live Drosophila æggestokkene væv

Article DOI: 10.3791/56316-v • 08:05 min • September 26th, 2017
September 26th, 2017 •
1, 1, 1
1Departments of Anatomy and OB/GYN-RS, University of California, San Francisco

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Vi leverer en protokol for imaging intracellulære pH af en epitel stamcelle afstamning i live Drosophila æggestokkene væv. Vi beskriver metoder til at generere transgene fluer udtrykker en pH biosensor, mCherry::pHluorin, billede biosensor ved hjælp af kvantitative fluorescens imaging, generere standard kurver og konvertere fluorescens intensitetsværdierne til pH-værdier.

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Udviklingsmæssige biologi sag 127 intracellulære pH follikel-stamceller epitelcelle Celledifferentiering pHluorin kvantitativ fluorescens imaging
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