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Journal / Cancer Research / 线粒体膜电位检测 CLIC4 击倒诱导 HN4…
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Cancer Research

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JoVE Journal Cancer Research
线粒体膜电位检测 CLIC4 击倒诱导 HN4 细胞凋亡的实验研究
Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis
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Journal (Cancer Research)
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线粒体膜电位检测 CLIC4 击倒诱导 HN4 细胞凋亡的实验研究

Article DOI: 10.3791/56317-v • 13:20 min • July 17th, 2018
July 17th, 2018 •
1, 2, 2, 2, 2, 2
1School of Basic Medical Sciences, Anhui Medical University, 2Department of Orthopedics, Anhui Provincial Hospital, Anhui Medical University

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在这里, 我们提出了一个详细的协议, 应用罗丹明123识别线粒体膜电位 (基质) 和研究 CLIC4 击倒诱导 HN4 细胞凋亡的体外。在普通荧光显微镜和共聚焦激光扫描荧光显微镜下, 记录了基质金属蛋白酶的实时变化。

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癌症研究 137 期 线粒体膜电位 罗丹明 123 共焦激光扫描荧光显微镜 氯离子通道 4 凋亡 头颈鳞癌
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