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Journal / Genetics / DNA 変更染色: 共焦点画像の数値解析
JoVE Journal
Genetics

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JoVE Journal Genetics
DNA 変更染色
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DNA 変更染色: 共焦点画像の数値解析

Article DOI: 10.3791/56318-v • 09:42 min • September 7th, 2017
September 7th, 2017 •
*1, *1, 1, 1, 1, 2, 2, 1, 3, 1
1Division of Cancer and Stem Cells, School of Medicine, Centre for Biomolecular Sciences, University of Nottingham, 2School of Life Sciences Imaging (SLIM), School of Life Sciences, University of Nottingham, 3Children's Brain Tumour Research Centre, School of Medicine, QMC, University of Nottingham
* These authors contributed equally

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新たに発見された 5-メチルシトシン (oxi mCs), 5-ヒドロキシメチルシトシン (5hmC), 5-formylcytosine (5 fC) の酸化フォームと 5-carboxylcytosine (5caC) は、独自の機能的役割の異なる DNA 変更を表す場合があります。ここの可視化から oxi mCs の空間分布、信号強度プロファイルとの共存の半定量的なワークフローを説明します。

Tags

遺伝学、問題 127、エピジェネティクス、DNA から oxi mCs、N6-メチルアデニン、免疫組織化学、禅、共焦点顕微鏡、信号の量子化、空間分布
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