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Journal / Genetics / Immunfarvning for DNA ændringer:…
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Genetics

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JoVE Journal Genetics
Immunfarvning for DNA ændringer
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Immunfarvning for DNA ændringer: beregningsmæssige analyse af Konfokal billeder

Article DOI: 10.3791/56318-v • 09:42 min • September 7th, 2017
September 7th, 2017 •
*1, *1, 1, 1, 1, 2, 2, 1, 3, 1
1Division of Cancer and Stem Cells, School of Medicine, Centre for Biomolecular Sciences, University of Nottingham, 2School of Life Sciences Imaging (SLIM), School of Life Sciences, University of Nottingham, 3Children's Brain Tumour Research Centre, School of Medicine, QMC, University of Nottingham
* These authors contributed equally

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Nyopdagede oxideret former for 5-methylcytosine (oxi-mCs), 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) og 5-carboxylcytosine (5caC) kan udgøre særskilte DNA ændringer med unikke funktionelle roller. Her er en semi-kvantitative arbejdsproces for visualisering af oxi-mCs' geografiske fordeling, signal intensitet profilering og colocalization beskrevet.

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Genetik sag 127 Epigenetik DNA oxi-mCs N6-methyladenine immunhistokemi Zen konfokalmikroskopi Signal kvantitering rumlige fordeling
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