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Journal / Genetics / Immunokleuring voor DNA wijzigingen:…
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Genetics

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JoVE Journal Genetics
Immunokleuring voor DNA wijzigingen
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Immunokleuring voor DNA wijzigingen: computationele analyse van Confocal beelden

Article DOI: 10.3791/56318-v • 09:42 min • September 7th, 2017
September 7th, 2017 •
*1, *1, 1, 1, 1, 2, 2, 1, 3, 1
1Division of Cancer and Stem Cells, School of Medicine, Centre for Biomolecular Sciences, University of Nottingham, 2School of Life Sciences Imaging (SLIM), School of Life Sciences, University of Nottingham, 3Children's Brain Tumour Research Centre, School of Medicine, QMC, University of Nottingham
* These authors contributed equally

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Onlangs ontdekte geoxideerde vormen van 5-methylcytosine (oxi-mCs), 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) en 5-carboxylcytosine (5caC) kan verschillende DNA wijzigingen met unieke functionele rollen. Hier wordt een semi-kwantitatieve workflow voor visualisatie van oxi-mCs ruimtelijke verdeling, signaal intensiteit profilering en colocalization omschreven.

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Genetica kwestie 127 epigenetica DNA oxi-mCs N6-methyladenine Immunohistochemistry Zen Confocal Microscopie signaal kwantificatie ruimtelijke verdeling
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