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Journal / Genetics / Immunostaining for DNA modifikasjoner:…
JoVE Journal
Genetics

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JoVE Journal Genetics
Immunostaining for DNA modifikasjoner
A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells
Journal (Genetics)
A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells
Raising the Mexican Tetra Astyanax mexicanus for Analysis of Post-larval Phenotypes and Whole-mount Immunohistochemistry
Journal (Genetics)
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Immunostaining for DNA modifikasjoner: beregningsorientert analyse av AC Confocal bilder

Article DOI: 10.3791/56318-v • 09:42 min • September 7th, 2017
September 7th, 2017 •
*1, *1, 1, 1, 1, 2, 2, 1, 3, 1
1Division of Cancer and Stem Cells, School of Medicine, Centre for Biomolecular Sciences, University of Nottingham, 2School of Life Sciences Imaging (SLIM), School of Life Sciences, University of Nottingham, 3Children's Brain Tumour Research Centre, School of Medicine, QMC, University of Nottingham
* These authors contributed equally

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Oppdagede nylig oksidert former for 5-methylcytosine (oxi-mCs), 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) og 5-carboxylcytosine (5caC) kan representere distinkte DNA endringer med unike funksjonsroller. Her beskrives en semi kvantitative arbeidsflyt for visualisering av oxi-mCs' romlige fordelingen, signalet intensitet profilering og colocalization.

Tags

Genetikk problemet 127 Epigenetics DNA oxi-mCs N6-methyladenine Immunohistochemistry Zen AC Confocal mikroskopi Signal kvantifisering romlige fordelingen
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