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JoVE Journal
Bioengineering

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化重构法 (的) 对肠道干细胞和小生境细胞功能分析的研究
 

化重构法 (的) 对肠道干细胞和小生境细胞功能分析的研究

Article doi: 10.3791/56329
November 20th, 2017

Summary November 20th, 2017

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肠道化文化是建立在整个棺, 不允许分析自我更新和分化的细胞特有的方式。本协议描述了对排序后的词干 (Lgr5+) 和小生境 (氏) 单元的重构, 这些细胞在进行生物化学和遗传修饰和功能分析之前, 会产生 organoids。

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