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JoVE Journal Cancer Research
Villkorliga Nedslagning av genuttryck i Cancer cellinjer att studera rekrytering av monocyter/makrofager att den tumör mikromiljö
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Villkorliga Nedslagning av genuttryck i Cancer cellinjer att studera rekrytering av monocyter/makrofager att den tumör mikromiljö

Article DOI: 10.3791/56333-v • 10:59 min • November 23rd, 2017
November 23rd, 2017 •
1,2, 1,2,3
1Division of Hematology, Oncology and Blood and Marrow Transplantation, Department of Pediatrics, University of Southern California, 2The Saban Research Institute of Children's Hospital Los Angeles, 3Department of Biochemistry and Molecular Medicine, University of Southern California

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Detta protokoll fungerar som ett system för att ställa in ett funktionellt Tet-ON system i cancer cellinjer och dess efterföljande användning, i synnerhet för studerar roll för tumör cell-derived proteiner i rekrytering av monocyter/makrofager att den tumör mikromiljö.

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Cancerforskning frågan 129 villkorad slå ner cellmigration doxycyklin shRNA PAI-1 monocyter cancer cellinjer
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