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JoVE Journal Cancer Research
Valutazione del processo di migrazione e l'invasione delle cellule
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Valutazione del processo di migrazione e l'invasione delle cellule: un confronto dello Scratch Video basati su microscopio ferita test e le analisi della camera di Boyden

Article DOI: 10.3791/56337-v • 11:20 min • November 17th, 2017
November 17th, 2017 •
*1,2, *1,2, 1,2, 1, 1,3, 1,3, 1,3, 1,2, 1,3, 1,2
1UMR CNRS 5822 /IN2P3, IPNL, PRISME, Laboratoire de Radiobiologie Cellulaire et Moléculaire, Faculté de Médecine Lyon-Sud, Université Lyon 1, 2Département de Radiothérapie, Institut de Cancérologie de la Loire - Lucien Neuwirth, 3Hospices Civils de Lyon, Centre Hospitalier Lyon-Sud
* These authors contributed equally

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Questo studio segnala due diversi metodi per l'analisi di migrazione e l'invasione delle cellule: l'analisi della camera di Boyden e l'in vitro dei video microscopio analisi basata a guarigione. Vengono descritti i protocolli per questi due esperimenti, e vengono confrontati i loro vantaggi e svantaggi.

Tags

Ricerca sul cancro problema 129 cameradi Boyden Scratch ferita invasione/migrazione motilità Video-microscopia chemiotassi cellulare
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