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Differentiering af musen embryonale stamceller i kortikale Interneuron prækursorer
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Differentiering af musen embryonale stamceller i kortikale Interneuron prækursorer

Article DOI: 10.3791/56358-v • 10:24 min • December 3rd, 2017
December 3rd, 2017 •
1,2, 1
1Neuroscience Graduate Group, Perelman School of Medicine, University of Pennsylvania, 2Department of Psychiatry, Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine

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Denne protokol beskriver en metode til at generere kortikale interneuron ophav og post mitotiske interneuron prækursorer fra mus embryonale stamceller ved hjælp af en modificeret embryoid krop til éncellelag metode. Disse stamfaderen/prækursorer kan være brugt i vitro fluorescently sorteret og transplanteret ind i neonatal neocortex for at studere skæbne bestemmelse eller anvendes til terapeutiske formål.

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Neurovidenskab spørgsmålet 130 hjernen mus interneuron differentiering cortex cellekultur stamceller transplantation
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