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JoVE Journal Neuroscience
Differensiering av mus embryonale stamceller i kortikale Interneuron forløpere
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Differensiering av mus embryonale stamceller i kortikale Interneuron forløpere

Article DOI: 10.3791/56358-v • 10:24 min • December 3rd, 2017
December 3rd, 2017 •
1,2, 1
1Neuroscience Graduate Group, Perelman School of Medicine, University of Pennsylvania, 2Department of Psychiatry, Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine

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Denne protokollen beskriver en metode for å generere kortikale interneuron progenitors og post mitotisk interneuron forløpere fra mus embryonale stamceller med en modifisert embryoid kroppen-å-monolayer-metode. Disse progenitors/forløpere kan være brukt i vitro fluorescently sortert og transplantert inn i neonatale neocortex for å studere skjebne bestemmelse eller brukes i terapeutiske programmer.

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Nevrovitenskap problemet 130 hjernen mus interneuron differensiering cortex cellekultur stamceller transplantasjon
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