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JoVE Journal Bioengineering
Division cellulaire chez les mammifères dans des Matrices 3D par microscopie Quantitative réflexion Confocal
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Division cellulaire chez les mammifères dans des Matrices 3D par microscopie Quantitative réflexion Confocal

Article DOI: 10.3791/56364-v • 10:22 min • November 29th, 2017
November 29th, 2017 •
*1,2, *1, 1, 1, 3, 1,2, 3, 1,2,4
1Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 2Johns Hopkins Physical Sciences - Oncology Center, Johns Hopkins University, 3Department of Biomedical Engineering, Johns Hopkins University, 4Departments of Oncology and Pathology and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine
* These authors contributed equally

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Ce protocole a efficacement étudie la division cellulaire chez les mammifères dans les matrices de collagène 3D en intégrant la synchronisation de la division cellulaire, la surveillance des manifestations de la division dans des matrices 3D à l’aide de la technique d’imagerie de cellules vivantes, microscopie de réflexion confocal résolution temporelle et analyse d’imagerie quantitative.

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Bio-ingénierie numéro 129 Division cellulaire synchronisation Interaction cellule-matrice matrice 3D microscopie confocale réflexion
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