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Bioengineering

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JoVE Journal Bioengineering
Divisione delle cellule dei mammiferi in matrici 3D tramite microscopia confocale quantitativa di riflessione
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Divisione delle cellule dei mammiferi in matrici 3D tramite microscopia confocale quantitativa di riflessione

Article DOI: 10.3791/56364-v • 10:22 min • November 29th, 2017
November 29th, 2017 •
*1,2, *1, 1, 1, 3, 1,2, 3, 1,2,4
1Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 2Johns Hopkins Physical Sciences - Oncology Center, Johns Hopkins University, 3Department of Biomedical Engineering, Johns Hopkins University, 4Departments of Oncology and Pathology and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine
* These authors contributed equally

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Questo protocollo studia in modo efficiente divisione cellulare dei mammiferi in matrici di collagene 3D integrando sincronizzazione della divisione cellulare, monitoraggio degli eventi di divisione in matrici 3D utilizzando la tecnica di imaging cellule vive, microscopia risolta in tempo di riflessione confocale e quantitative analisi di imaging.

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Bioingegneria problema 129 divisione cellulare Matrix 3D sincronizzazione interazione cellula-matrice microscopia Confocal di riflessione
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