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JoVE Journal Bioengineering
Pattedyr celledeling i 3D matriser via kvantitative AC Confocal refleksjon mikroskopi
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Pattedyr celledeling i 3D matriser via kvantitative AC Confocal refleksjon mikroskopi

Article DOI: 10.3791/56364-v • 10:22 min • November 29th, 2017
November 29th, 2017 •
*1,2, *1, 1, 1, 3, 1,2, 3, 1,2,4
1Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 2Johns Hopkins Physical Sciences - Oncology Center, Johns Hopkins University, 3Department of Biomedical Engineering, Johns Hopkins University, 4Departments of Oncology and Pathology and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine
* These authors contributed equally

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Denne protokollen effektivt studier pattedyr celledeling i 3D kollagen matriser ved å integrere synkronisering av cellen divisjon, overvåking av løpene i 3D matriser med live-celle tenkelig teknikk, tid-løst AC confocal refleksjon mikroskopi og kvantitativ tenkelig analyse.

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Bioteknologi problemet 129 celledeling 3D Matrix synkronisering celle-Matrix samhandling AC Confocal refleksjon mikroskopi
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