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JoVE Journal
Bioengineering

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超薄 Porated 弹性水凝胶作为双细胞培养的仿生基底膜
 
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超薄 Porated 弹性水凝胶作为双细胞培养的仿生基底膜

Article DOI: 10.3791/56384-v 11:34 min December 26th, 2017
December 26th, 2017

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Summary

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目前的双层培养模式不允许功能性的体外研究, 模仿体内环境。使用聚乙二醇和氧化锌模板化方法, 该协议描述了一个超薄仿生基底膜的发展, 具有可调谐的刚度, 孔隙率和生物化学组成, 密切模仿在体内细胞外基质。

Tags

生物工程 问题 130 生物材料 基底膜 聚乙二醇 微孔 双层 弹性水凝胶
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